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KMID : 0364820090450030281
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Korean Journal of Microbiology
2009 Volume.45 No. 3 p.281 ~ p.285
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Evaluation of Liquid Culture System in Sputum Culture and Drug Susceptibility of Mycobacterium
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Kim Jin-Suk
Kim Seung-Chul
Jeon Bo-Young
Park Seung-Kyu
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Abstract
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In this study, we compared the BacT/Alert liquid culture system with Ogawa and Lowenstein-Jensen (L-J) media for sputum culture and drug susceptibility test (DST) of Mycobacterium tuberculosis. Rapid liquid culture systems have been widely employed both for primary cultures of M. tuberculosis from clinical specimens and for drug susceptibility test because of its greater sensitivity and faster turn-around time than the conventional egg-based culture methods (Ogawa, Lowenstein-Jensen media). Sputum specimens were decontaminated with N-acetyl-L-cysteine (NALC)-4% NaOH and inoculated into the BacT/Alert culture bottles and Ogawa media. 95 from among 135 sputa were smear-positive, 97 (71.9%) were culture-positive by the BacT/Alert culture system, while 89 (65.9%) were positive by Ogawa media. The mean time to culture-positive by the BacT/Alert process system was about 11.3 days, which was significantly shorter than that by Ogawa media (22.4 days). Of 32 M. tuberculosis cultures examined for drug sensitivity, the concordant rate between the two methods (BacT/Alert liquid culture system, Lowenstein-Jensen media) ranged from 87.5% for isoniazid and 90.6% for rifampicin.
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KEYWORD
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acid fast bacilli (AFB), BacT/alert process system, isoniazid (INH), M. tubercilosis, rifampicin (RIF), sputum culture, drug susceptibility test (DST)
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